The laboratory of Dr. Bruce E. Torbett is looking for three Staff Scientists to be respective workstream leaders in the following projects:
1- Lentiviral Vector Engineering and Improved Gene Delivery to Hematopoietic Stem Cells (Project ID 2021-40233)
This translational work aims to improve the delivery of therapeutic genes and gene-modifying agents for the purpose of genetic correction as well as the prevention of viral infections. The candidate will be involved in setting up and managing studies to determine restrictions of lentiviral vector (LV) entry to HSCs and how they can be overcome by using small molecule transduction enhancers (PMID 24914132, 31416800), alternate surface envelopes (PMID 30603655), or engineering improved producer cell lines. Candidates must have a Ph.D. in Cell/Molecular Biology related field with documented experience in production and use of lentiviral vectors in primary cells, preclinical testing of LV gene therapy in humanized mouse models, as well as familiarity with standard virology techniques.
Basic Qualifications: lentiviral (LV) vector production and gene delivery to primary cells, preclinical testing of LV vectors in humanized mouse models, standard virology assays (neutralization, titer, packaging efficiency), multicolor flow cytometry analysis and cell sorting, cell culture of primary cells and cell lines, RT-qPCR, protein and nucleic acid isolation and analysis.
Preferred Qualifications: immunoassays (eg. p24 ELISA), gene targeting with CRISPR/Cas9 or equivalent, experience working in BSL2+ and BSL3 biocontainment environments, fluorescence microscopy.
2- Immune Signaling Pathways involved in HIV entry (Project ID 2021-40231)
This work aims to investigate how opioids (and other immune modulators) affect HIV entry and latency, with particular focus on signaling pathways involved in host restriction and viral reactivation. This work also includes focus on the molecular mechanisms for HIV transport through the Nuclear Pore Complex, with insights of novel host-viral interfaces being translatable to new therapeutic strategies. This work involves setting up and managing studies of immune signaling pathways during active HIV infection in primary cells using Molecular Virology approaches. Candidates must have a Ph.D. in Virology or related field, with documented experience using standard virology approaches to investigate immune-signaling dynamics.
Basic Qualifications: molecular virology, isolation and culture of primary cells, fluorescence microscopy, various methods of protein isolation and qualification and quantification (including immunoassays), gene-targeting approaches (such as KOs and knockdowns).
Preferred Qualifications: flow cytometry and FACS, use of viral vectors for heterologous gene expression or gene targetting, experience working in BSL2+ and BSL3 biocontainment environments, confocal microcopy (or other high-resolution microscopy).
3- Structural and Biophysical Interactions of HIV Gag/Gag-Pol with host proteins during viral trafficking and assembly (Project ID 2021-40232)
This work aims to investigate how HIV co-opts cellular host machinery during active infection and identify the composition, dynamics, and function of novel host-viral interfaces that can be modulated for new therapeutic strategies (PMID 33807824). This work involves setting up and managing studies utilizing proximity labeling and quantitative proteomics, along with biochemical and virological validation of candidate cellular factors using CRISPR/Cas9 knockouts and confocal imaging techniques. Candidates must have a Ph.D. in Biochemistry or related field with documented experience in Mass Spectrometry-based Proteomics to identify host-factors of microbial infections, along with experience in target validation using relevant in vivo and in vitro assays.
Basic Qualifications: mass spectrometry-based proteomics to identify host-factors of microbial infections, target validation using relevant in vivo and in vitro assays (eg. CRISPR/Cas9), cell culture, fluorescence microscopy, various methods of protein isolation/labeling/qualification/quantification, bioconjugation and/or chemical biology.
Preferred Qualifications: working knowledge of HTS technologies, Immunoassays (such as p24 ELISA), knowledge of structural biology, confocal microscopy, knowledge of bioinformatics as it pertains to analysis of proteomic datasets, protein engineering.
GENERAL JOB RESPONSIBILITIES
- Implement and run a workstream in an autonomous manner in close coordination with Principal Investigator
- Development/Writing of Grants and Funding Opportunities
- Meet with Principal Investigator on a regular basis to discuss proposed/ongoing studies, discuss implications of findings, and provide insights into new research directions
- Within the candidate’s area of expertise, actively support Principal Investigator in training and supervising of Post-Doctoral Fellows, Graduate Students, and Technicians
- Run research projects in an independent manner
- Write manuscripts, present data at national and international conferences
- Assist PI in writing progress reports, grant proposals, protocols
- Protocol development and review
- Excellent communication and writing skills
- Develop additional scientific skills as appropriate for the research project and carry out research/development activities of assigned research projects.
- Ability to learn and apply new technologies and skills
INSTRUCTIONS: Interested candidates please e-mail firstname.lastname@example.org directly and include CV, three references, and a Cover Letter. Please include Project ID in the Subject Line